Details

Autor(en) / Beteiligte

• Pan, X-X
• Wang, J
• Xia, W-Y
• Li, X-F
• Yang, L-J
• Huang, C
• Chen, Y-D

Titel

Immunogenicity and antigenicity of a recombinant chimeric protein containing epitopes of poliovirus type 1

Ist Teil von

• Acta virologica (Anglickâa verze), 2016, Vol.60 (3), p.234-241

Ort / Verlag

Slovakia

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• To design a vaccine that simultaneously prevents both rotavirus (RV) and poliovirus (PV), a PV type 1 (PV1) chimeric protein using RV VP6 as a vector (VP6F) was constructed, expressed in Escherichia coli expression system and characterized by SDS-PAGE, Western blot, immunofluorescence assay and neutralization test. The results showed that the chimeric protein reacted with anti-VP6F and anti-PV1 antibodies and elicited production of serum antibodies against the chimeric protein in guinea pigs. Antibodies against the chimeric protein neutralized RV Wa and PV1 infection in vitro. The results provided a relevant possibility of developing novel approaches in the rational design of vaccines effective against both RV and PV.