Details

Autor(en) / Beteiligte

• Nishizawa-Yokoi, Ayako
• Saika, Hiroaki
• Toki, Seiichi

Titel

Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2016-01, Vol.1469, p.137

Ort / Verlag

United States

Erscheinungsjahr

2016

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Positive-negative selection using hygromycin phosphotransferase (hpt) and diphtheria toxin A-fragment (DT-A) as positive and negative selection markers, respectively, allows enrichment of cells harboring target genes modified via gene targeting (GT). We have developed a successful GT system employing positive-negative selection and subsequent precise marker excision via the piggyBac transposon derived from the cabbage looper moth to introduce desired modifications into target genes in the rice genome. This approach could be applied to the precision genome editing of almost all endogenous genes throughout the genome, at least in rice.