Details

Autor(en) / Beteiligte

• Zhang, W
• Wu, Y
• Gong, L
• Zhu, H L
• Huang, G C
• Zhou, G Q
• Wu, K F
• Liu, D S

Titel

Therapeutic effect of IGF-1R-targeting inhibitor (TAE226) on malignant pleural effusion in nude mice

Ist Teil von

• Zhōnghuá zhŏngliú zázhì, 2016-08, Vol.38 (8), p.565

Ort / Verlag

China

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• To study the therapeutic effect of IGF-1R inhibitor TAE226 on malignant pleural effusion (MPE) in nude mice. Human lung carcinoma A549 cells were injected into the pleural cavity of nude mice to establish MPE model. The mice were randomly divided into model group and treatment group, and were orally administered with distilled water and TAE226 (20 mg/kg) in the same volume, respectively. The volume of pleural effusion and tumor weight of the two groups were observed. HE staining was used to reveal the histological changes and enzyme-linked immunosorbent assay (ELISA) was used to detect the IGF-1R protein expression. IGF-1R mRNA level in the tumor tissue was determined by RT-PCR. Microvessel density (MVD) and cell proliferation index (PI) were assessed by immunohistochemical analysis. The protein expression levels of IGF-1R, p-IGF-1R, PI3K and p-PI3K in the tumor tissue were determined by Western blotting. The volumes of pleural effusion were (241.4±89.7) μl and (121.7±78.8) μl in the model and treatment group