Details
- Autor(en) / Beteiligte
- Titel
- Effect of GTPase activating protein Git2 on metastasis in breast cancer
- Ist Teil von
- Zhōnghuá zhŏngliú zázhì, 2016-07, Vol.38 (7), p.492
- Ort / Verlag
- China
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To investigate the effect of GTPase activating protein Git2 on metastasis in breast cancer. Git2 gene over-expression was induced by Git2 cDNA, and Git2 gene knockdown was induced by Git2 ShRNA lentivirus in four breast cancer cell lines. Six-week old wide type female mice were also used in this study. The cells were tagged with luciferase and injected into wide type female mice by tail vein or 4(th) mammary fat pad, respectively, to establish a cancer metastasis model. In vivo real time imaging system and immunohistochemical staining were used to detect the cancer metastasis. The relative mRNA expression level of Git2 (normalized by GAPDH) in the 4T1, 4TO7, 168FARN and 67NR cells were 0.91±0.03, 0.125±0.06, 0.131±0.04 and 0.92±0.04, respectively. The expression of EMT marker E-cadherin was inhibited and N-cadherin and vimentin were enhanced when Git2 was over-expressed in 168FARN cells and 4TO7 cells expressing low level of Git2, whereas the expression of E-cadherin was increased and N-cadherin and vimentin
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0253-3766DOI: 10.3760/cma.j.issn.0253-3766.2016.07.003Titel-ID: cdi_pubmed_primary_27531261
- Format
- –
- Schlagworte
- Animals, Breast Neoplasms - genetics, Breast Neoplasms - pathology, Cadherins - metabolism, Cell Line, Tumor, Down-Regulation, Female, Gene Expression, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, GTPase-Activating Proteins - genetics, GTPase-Activating Proteins - metabolism, Humans, Lentivirus - genetics, Luciferases, Lung Neoplasms - secondary, Mice, Mice, Inbred BALB C, Neoplasm Metastasis, RNA, Messenger - metabolism, RNA, Small Interfering, Vimentin - metabolism