Details

Autor(en) / Beteiligte

• Ye, San-xian
• Luo, Yun-jing
• Qiao, Shu-liang
• Li, Li
• Liu, Cai-hong
• Shi, Jian-long
• An, Xue-jing

Titel

Study of Reaction Dynamics between Bovine Serum Albumin and Folic Acid by Stopped-Flow/Fluorescence

Ist Teil von

• Guang pu xue yu guang pu fen xi, 2016-01, Vol.36 (1), p.134

Ort / Verlag

China

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• As a kind of coenzyme of one-carbon enzymes in vivo, folic acid belongs to B vitamins, which can interact with other vitamins and has great significance for converting among amino acids, dividing growth of cells and protein synthesis reactions. Half-life, concentration and reaction rate constant of drugs are important parameters in pharmacokinetic study. In this paper, by utilizing fluorescence spectrophotometer and stopped-flow spectrum analyzer, reaction kinetic parameters between bovine serum albumin(BSA) and folic acid in a bionic system have been investigated, which provide references for parameters of drug metabolism related to folic acid. By using Stern-Volmer equation dealing with fluorescence quenching experiments data, we concluded that under 25, 30, and 37 degrees C, the static quenching constants of folic acid to intrinsic fluorescence from bovine serum albumin were 2.455 x 10(10), 4.900 x 10(10) and 6.427 x 10(10) L x mol(-1) x s(-1) respectively; The results of kinetic reaction rate have shown t