Details

Autor(en) / Beteiligte

• Fan, B
• Yin, Y S
• Sun, G
• Zhu, L Y
• Liu, W
• Pi, X E
• Fei, D B
• Peng, L H
• Wang, X
• Yang, Y S

Titel

Effects of different carbohydrates on the simulation of human intestinal bacterial flora with in vitro culture

Ist Teil von

• Chung-hua nei kʿo tsa chih, 2016-05, Vol.55 (5), p.381

Ort / Verlag

China

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the optimal growth condition of human fecal bacterial flora in vitro by comparing the effect of different carbohydrates as cultural media. Three fecal samples (1, 2, 3) were collected and inoculated into a single-stage chemostat system, in which starch medium (VI) and starch polysaccharide medium(XP) were used. Samples were collected for bacterial genomic DNA extraction and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis. Bacterial composition and short chain fatty acid (SCFA) were then analyzed. The single stage chemostat system reached steady after operating 8 days, when evaluated by the PCR-DGGE. Bacterial 16s rRNA high-throughput sequencing showed that the intestinal bacteria of these three volunteers was mainly composed of four bacterial phyla, namely, Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria. When the influence of bacterial abundance was considered, the similarity of bacterial composition between the original fecal samples to the h