Nature immunology, 2016-05, Vol.17 (5), p.538
2016
Details
- Autor(en) / Beteiligte
- Titel
- Acidic chitinase primes the protective immune response to gastrointestinal nematodes
- Ist Teil von
- Nature immunology, 2016-05, Vol.17 (5), p.538
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Acidic mammalian chitinase (AMCase) is known to be induced by allergens and helminths, yet its role in immunity is unclear. Using AMCase-deficient mice, we show that AMCase deficiency reduced the number of group 2 innate lymphoid cells during allergen challenge but was not required for establishment of type 2 inflammation in the lung in response to allergens or helminths. In contrast, AMCase-deficient mice showed a profound defect in type 2 immunity following infection with the chitin-containing gastrointestinal nematodes Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. The impaired immunity was associated with reduced mucus production and decreased intestinal expression of the signature type 2 response genes Il13, Chil3, Retnlb, and Clca1. CD103(+) dendritic cells, which regulate T cell homing, were also reduced in mesenteric lymph nodes of infected AMCase-deficient mice. Thus, AMCase functions as a critical initiator of protective type 2 responses to intestinal nematodes but is largely dispensable for allergic responses in the lung.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1529-2916DOI: 10.1038/ni.3417Titel-ID: cdi_pubmed_primary_27043413
- Format
- –
- Schlagworte
- Animals, beta-N-Acetylhexosaminidases - genetics, beta-N-Acetylhexosaminidases - immunology, beta-N-Acetylhexosaminidases - metabolism, Chitinases - genetics, Chitinases - immunology, Chitinases - metabolism, Chloride Channels - genetics, Chloride Channels - immunology, Chloride Channels - metabolism, Flow Cytometry, Gastrointestinal Tract - immunology, Gastrointestinal Tract - metabolism, Gastrointestinal Tract - parasitology, Gene Expression - immunology, Hormones, Ectopic - genetics, Hormones, Ectopic - immunology, Hormones, Ectopic - metabolism, Host-Parasite Interactions - immunology, Hypersensitivity - genetics, Hypersensitivity - immunology, Hypersensitivity - metabolism, Immunity - genetics, Immunity - immunology, Interleukin-13 - genetics, Interleukin-13 - immunology, Interleukin-13 - metabolism, Lectins - genetics, Lectins - immunology, Lectins - metabolism, Lung - immunology, Lung - metabolism, Lung - pathology, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Fluorescence, Nematospiroides dubius - immunology, Nematospiroides dubius - physiology, Nippostrongylus - immunology, Nippostrongylus - physiology, Reverse Transcriptase Polymerase Chain Reaction, Strongylida Infections - immunology, Strongylida Infections - metabolism, Strongylida Infections - parasitology