Details

Autor(en) / Beteiligte

• Wang, Song-bai
• Zhang, Yan
• An, Wen-ting
• Wei, Yan-li
• Wang, Yu
• Shuang, Shao-min

Titel

Fluorescent and Magnetic Relaxation Switch Immunosensor for the Detecting Foodborne Pathogen Salmonella enterica in Water Samples

Ist Teil von

• Guang pu xue yu guang pu fen xi, 2015-11, Vol.35 (11), p.3105

Ort / Verlag

China

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Fluoroimmunoassay based on quantum dots (QDs) and magnetic relaxation switch (MRS) immunoassay based on superparamagnetic nanoparticles (SMN) were constructed to detect Salmonella enterica (S. enterica) in water samples. In fluoroimmunoassay, magnetic beads was conjugated with S. enterica capture antibody (MB-Ab2) to enrich S. enterica from sample solution, then the QDs was conjugated with the S. enterica detection antibody (QDs-Ab1) to detect S. enterica based on sandwich immunoassay format. And the fluorescence intensity is positive related to the bacteria concentration of the sample. Results showed that the limit of detection (LOD) of this method was 102 cfu · mL⁻¹ and analysis time was 2 h. In MRS assay, magnetic nanoparticle-antibody conjugate (MN-Ab1) can switch their dispersed and aggregated state in the presence of the target. This state of change can modulate the spin-spin relaxation time (T₂) of the neighboring water molecule. The change in T₂(ΔT₂) positively correlates with the amount of the target