Details

Autor(en) / Beteiligte

• Wheeler, Michael A
• Smith, Cody J
• Ottolini, Matteo
• Barker, Bryan S
• Purohit, Aarti M
• Grippo, Ryan M
• Gaykema, Ronald P
• Spano, Anthony J
• Beenhakker, Mark P
• Kucenas, Sarah
• Patel, Manoj K
• Deppmann, Christopher D
• Güler, Ali D

Titel

Genetically targeted magnetic control of the nervous system

Ist Teil von

• Nature neuroscience, 2016-05, Vol.19 (5), p.756

Ort / Verlag

United States

Erscheinungsjahr

2016

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Optogenetic and chemogenetic actuators are critical for deconstructing the neural correlates of behavior. However, these tools have several limitations, including invasive modes of stimulation or slow on/off kinetics. We have overcome these disadvantages by synthesizing a single-component, magnetically sensitive actuator, "Magneto," comprising the cation channel TRPV4 fused to the paramagnetic protein ferritin. We validated noninvasive magnetic control over neuronal activity by demonstrating remote stimulation of cells using in vitro calcium imaging assays, electrophysiological recordings in brain slices, in vivo electrophysiological recordings in the brains of freely moving mice, and behavioral outputs in zebrafish and mice. As proof of concept, we used Magneto to delineate a causal role of striatal dopamine receptor 1 neurons in mediating reward behavior in mice. Together our results present Magneto as an actuator capable of remotely controlling circuits associated with complex animal behaviors.