Nature neuroscience, 2016-04, Vol.19 (4), p.578
2016
Details
- Autor(en) / Beteiligte
- Titel
- Fluorescent false neurotransmitter reveals functionally silent dopamine vesicle clusters in the striatum
- Ist Teil von
- Nature neuroscience, 2016-04, Vol.19 (4), p.578
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Neurotransmission at dopaminergic synapses has been studied with techniques that provide high temporal resolution, but cannot resolve individual synapses. To elucidate the spatial dynamics and heterogeneity of individual dopamine boutons, we developed fluorescent false neurotransmitter 200 (FFN200), a vesicular monoamine transporter 2 (VMAT2) substrate that selectively traces monoamine exocytosis in both neuronal cell culture and brain tissue. By monitoring electrically evoked Ca(2+) transients with GCaMP3 and FFN200 release simultaneously, we found that only a small fraction of dopamine boutons that exhibited Ca(2+) influx engaged in exocytosis, a result confirmed with activity-dependent loading of the endocytic probe FM1-43. Thus, only a low fraction of striatal dopamine axonal sites with uptake-competent VMAT2 vesicles are capable of transmitter release. This is consistent with the presence of functionally 'silent' dopamine vesicle clusters and represents, to the best of our knowledge, the first report suggestive of presynaptically silent neuromodulatory synapses.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1546-1726DOI: 10.1038/nn.4252Titel-ID: cdi_pubmed_primary_26900925
- Format
- –
- Schlagworte
- Animals, Cells, Cultured, Corpus Striatum - chemistry, Corpus Striatum - metabolism, Dopamine - analysis, Dopamine - metabolism, Exocytosis - physiology, Female, Fluorescent Dyes - analysis, Fluorescent Dyes - metabolism, HEK293 Cells, Humans, Male, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Neurotransmitter Agents - analysis, Neurotransmitter Agents - metabolism, Organ Culture Techniques, Presynaptic Terminals - chemistry, Presynaptic Terminals - metabolism, Synaptic Vesicles - chemistry, Synaptic Vesicles - metabolism