Methods in molecular biology (Clifton, N.J.), 2016, Vol.1350, p.95
2016
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Details
- Autor(en) / Beteiligte
- Titel
- Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2016, Vol.1350, p.95
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. BacMams have become an integral part of the recombinant mammalian gene expression toolbox in research labs worldwide. Construction of transfer vectors is straightforward using basic molecular biology protocols. Virus generation is based on common methods used with the baculovirus insect cell expression system. BacMam transduction of mammalian cells requires minimal modifications to familiar cell culture methods. This chapter highlights the BacMam transfer vector pHTBV.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-4939-3043-2_5Titel-ID: cdi_pubmed_primary_26820855
- Format
- –
- Schlagworte
- Animals, Baculoviridae - genetics, Baculoviridae - isolation & purification, CHO Cells, Cloning, Molecular, Cricetinae, Cricetulus, DNA Restriction Enzymes - genetics, DNA, Complementary - genetics, Gene Expression, Genetic Engineering - methods, Genetic Vectors - genetics, HEK293 Cells, Humans, Nucleic Acid Amplification Techniques, Sf9 Cells, Spodoptera, Transformation, Genetic