Nature methods, 2016-03, Vol.13 (3), p.233-236
2016
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Details
- Autor(en) / Beteiligte
- Titel
- Predictable tuning of protein expression in bacteria
- Ist Teil von
- Nature methods, 2016-03, Vol.13 (3), p.233-236
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/nmeth.3727Titel-ID: cdi_pubmed_primary_26752768
- Format
- –
- Schlagworte
- Algorithms, Cloning, Molecular, Escherichia coli - genetics, Escherichia coli Proteins - genetics, Peptide Chain Initiation, Translational - genetics, Promoter Regions, Genetic - genetics, Protein Engineering - methods, RNA, Bacterial - genetics, RNA, Messenger - genetics, RNA, Ribosomal, 16S - genetics, Sequence Homology, Nucleic Acid, Software