Details

Autor(en) / Beteiligte

• Yang, Luhan
• Güell, Marc
• Niu, Dong
• George, Haydy
• Lesha, Emal
• Grishin, Dennis
• Aach, John
• Shrock, Ellen
• Xu, Weihong
• Poci, Jürgen
• Cortazio, Rebeca
• Wilkinson, Robert A.
• Fishman, Jay A.
• Church, George

Titel

Genome-wide inactivation of porcine endogenous retroviruses (PERVs)

Ist Teil von

• Science (American Association for the Advancement of Science), 2015-11, Vol.350 (6264), p.1101-1104

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

2015

Link zum Volltext

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• The shortage of organs for transplantation is a major barrier to the treatment of organ failure. Although porcine organs are considered promising, their use has been checked by concerns about the transmission of porcine endogenous retroviruses (PERVs) to humans. Here we describe the eradication of all PERVs in a porcine kidney epithelial cell line (PK15). We first determined the PK15 PERV copy number to be 62. Using CRISPR-Cas9, we disrupted all copies of the PERV pol gene and demonstrated a >1000-fold reduction in PERV transmission to human cells, using our engineered cells. Our study shows that CRISPR-Cas9 multiplexability can be as high as 62 and demonstrates the possibility that PERVs can be inactivated for clinical application of porcine-to-human xenotransplantation.