Nature methods, 2015-12, Vol.12 (12), p.1135-1138
2015
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Details
- Autor(en) / Beteiligte
- Titel
- A strategy for dissecting the architectures of native macromolecular assemblies
- Ist Teil von
- Nature methods, 2015-12, Vol.12 (12), p.1135-1138
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/nmeth.3617Titel-ID: cdi_pubmed_primary_26436480
- Format
- –
- Schlagworte
- Animals, Cross-Linking Reagents - chemistry, Electrophoresis, Polyacrylamide Gel, Exosomes - chemistry, Green Fluorescent Proteins - genetics, Liver - chemistry, Mass Spectrometry - methods, Mice, Transgenic, Models, Molecular, Multiprotein Complexes - chemistry, Multiprotein Complexes - genetics, Protein Conformation, Saccharomyces cerevisiae - chemistry, Saccharomyces cerevisiae Proteins - chemistry, Sensitivity and Specificity