Details

Autor(en) / Beteiligte

• Muellener, D
• Rudin, O
• Pauli, U
• Zink, P

Titel

Sex determination of human blood micro-stains and single hairs using specific DNA amplification with polymerase chain reaction

Ist Teil von

• Archiv fur Kriminologie, 1989-11, Vol.184 (5-6), p.175

Ort / Verlag

Germany

Erscheinungsjahr

1989

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Amplification of Y chromosome specific DNA in vitro enables a rapid and reliable sex determination of human minute traces such as blood stains and hairs. In presence of male DNA a band of 154 bp is visualized by agarose gel electrophoresis after amplification, this band is lacking in case of female DNA alone. Amplification of a sex independent DNA locus (such as a fragment from the alcohol dehydrogenase gene) generates identical reaction products for both sexes. This shows that the absence of a band is not due to the lack of trace DNA. It is possible to perform this technique with as little as 0.5 microliters of blood or with a single hair.