Ukrainian biochemical journal, 2015-03, Vol.87 (2), p.66
2015
Details
- Autor(en) / Beteiligte
- Titel
- PECULIARITIES OF GLUCOSE AND GLYCEROL METABOLISM IN Nocardia vaccinii IMB B-7405
- Ist Teil von
- Ukrainian biochemical journal, 2015-03, Vol.87 (2), p.66
- Ort / Verlag
- Ukraine
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- It has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NAD+-dependent glucose-6-phosphate dehydrogenase and FAD+-dependent glucose dehydrogenase 835 ± 41 and 698 ± 35 nmol.min-1.mg-1 of protein respectively). 6-Phosphogluconate formed in the gluconokinase reaction is involved in the pentose phosphate cycle (activity of constitutive NADP+-dependent 6-phosphogluconate dehydrogenase 357 ± 17 nmol.min-1.mg-1 of protein). Glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 244 ± 12 nmol.min-1.mg-1 of protein) and through dihydroxyacetone. Replenishment of the C4-dicarboxylic acids pool in N. vaccinii IMV B-7405 grown on glucose and glycerol occurs in the phosphoenolpyruvate(PEP)carboxylase reaction (714-803 nmol.min-1.mg-1 of protein). 2-Oxoglutarate was involved in tricarboxylic acid cycle by alternate pathway with the participation of 2-oxoglutarate synthase. The observed activity of both key enzymes of gluconeogenesis (PEP-carboxykinase and PEP-synthase), trehalose phosphate synthase and NADP+-dependent glutamate dehydrogenase confirmed the ability of IMV B-7405 strain to the synthesis of surface active glycoand aminolipids, respectively.
- Sprache
- Ukrainisch
- Identifikatoren
- ISSN: 2409-4943DOI: 10.15407/ubj87.02.066Titel-ID: cdi_pubmed_primary_26255340
- Format
- –
- Schlagworte
- Bacterial Proteins - metabolism, Citric Acid Cycle - physiology, Dihydroxyacetone - metabolism, Dihydroxyacetone Phosphate - metabolism, Gluconates - metabolism, Gluconeogenesis - physiology, Glucose - metabolism, Glucose 1-Dehydrogenase - metabolism, Glucosephosphate Dehydrogenase - metabolism, Glutamate Dehydrogenase (NADP+) - metabolism, Glycerol - metabolism, Glycerophosphates - metabolism, Glycolysis - physiology, Ketoglutaric Acids - metabolism, Ketone Oxidoreductases - metabolism, Nocardia - metabolism, Pentose Phosphate Pathway - physiology, Phosphoenolpyruvate - metabolism, Phosphoenolpyruvate Carboxykinase (ATP) - metabolism, Phosphoenolpyruvate Carboxylase - metabolism, Phosphogluconate Dehydrogenase - metabolism, Phosphotransferases (Alcohol Group Acceptor) - metabolism, Phosphotransferases (Paired Acceptors) - metabolism