Details

Autor(en) / Beteiligte

• Long, Zhang-Biao
• Huang, Jian-Song
• Shi, Xiao-Feng
• Yang, Ji-Chun
• Ruan, Zheng
• Xiao, Bing
• Xi, Xiao-Dong

Titel

Myr-RKEFAK Peptide Selectively Regulates Outside-in Signaling Transduction-related Functions in Human Platelets

Ist Teil von

• Zhongguo shi yan xue ye xue za zhi, 2015-06, Vol.23 (3), p.761

Ort / Verlag

China

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• To study the effect of interaction of the talin rod domain integrin binding site 2 with integrin β3 on platelet signal transduction. A peptide that mimics the membrane proximal α helix 6 residues R724 KEFAK729 of the integrin β3 cytoplasmic tails was designed and synthesized, to which the myristoylation was covalently linked to the N-terminal of the peptide enabling membrane penetration. The effects of myr-RKEFAK peptide on the typical platelet outside-in signaling ovent (stable adhesion and spreading on immobilized fibrinogen, aggregation, fibrin clot retraction) and inside-out signaling events (soluble fibrinogen binding) were tested. myr-RKEFAK peptide dose-dependently inhibited platelet stable adhesion and spreading on immobilized fibrinogen, irreversible aggregation, as well as fibrin clot retraction, but not soluble fibrinogen binding and reversible phase of platelet aggregation. The cell-penetrating peptide myr-RKEFAK causes an inhibitory effect on integrin β3 outside-in signaling-regulated platelets f