Details

Autor(en) / Beteiligte

• Shi, Guoxiang
• Zhang, Zheng
• Mei, Lingling
• Chen, Jinhua
• Mei, Shenghua
• Jin, Dazhi
• Zhang, Zhikai
• Wang, Yumeng
• Zhang, Xiaohe
• Luo, Yun
• Sun, Jimin
• Yu, Dongzheng
• Xia, Lianxu

Titel

Establishment and evaluation of identification method for Yersinia pestis and Yersinia pseudotuberculosis

Ist Teil von

• Zhōnghuá liúxíngbìng zázhì, 2015-05, Vol.36 (5), p.496

Ort / Verlag

China

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• To establish a gene identification method of Yersinia pestis and Yersinia pseudotuberculosis for plague surveillance. According to the specific genomic sequences of Y. pestis and Y. pseudotuberculosis, i.e. "pestis Island (PeI)" and "pseudotuberculosis Island (PsI)" and the published genomic sequences of 12 strains of Y. pestis and 4 strains of Y. pseudotuberculosis, the specific identification primers of these sequences were designed. A total of 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis and other intestinal bacteria strains were tested with PCR. Of the 5 pairs of Y. pestis identification primers, PeI2 and PeI11 were specific for Y. pestis. Besides Y. pestis, the primers PeI1, PeI3 and PeI12 could detect part of 57 Y. pseudotuberculosis strains. Of the 5 pairs of Y. pseudotuberculosis identification primers, PsI1 could detect all the 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis. PsI7, PsI16, PsI18 and PsI19 were specific for Y. pseudotuberculosis. The primers PsI1, PeI