Details

Autor(en) / Beteiligte

• Yi, Zhijuan
• Hao, Guijie
• Yuan, Xuemei
• Pan, Xiaoyi
• Xu, Yang
• Yao, Jiayun
• Lin, Lingyun
• Yin, Wenlin
• Shen, Jinyu

Titel

Inactivated vaccine for hemorrhage of Grass carp up-regulates the expressions of major immune-related genes in spleen of Grass carp

Ist Teil von

• Xi bao yu fen zi mian yi xue za zhi, 2015-02, Vol.31 (2), p.177

Ort / Verlag

China

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the impact of the injection of inactivated vaccine for hemorrhage of Grass carp on the expressions of main immune-related genes in spleen including immunoglobulin M (IgM), major histocompatibility complexI(MHC I), interferon I(IFN1), complement 3 (C3), interleukin-1β (IL-1β) and intelectin genes. Ctenopharyngoden idellus kidney (CIK) cells were inoculated with Grass carp reovirus (GCRV). Then inactivated vaccine was prepared by inactivating virus suspension with formaldehyde. Vaccine was 50- and 100-times diluted with normal saline. The experiments included four groups: undiluted group, 50-times diluted group, 100-times diluted group and normal saline control group. Then healthy Grass carps with the average body mass (20 ± 5) g were intraperitoneally injected with vaccine or normal saline separately for each group. Spleens were collected at different time points after injection (0, 6, 12, 24, 72 hours) and total RNA extraction was performed immediately. Real-time quantitative PCR (qRT-PCR) was