Nature methods, 2015-03, Vol.12 (3), p.199-202
2015
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Real-time deformability cytometry: on-the-fly cell mechanical phenotyping
- Ist Teil von
- Nature methods, 2015-03, Vol.12 (3), p.199-202
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/nmeth.3281Titel-ID: cdi_pubmed_primary_25643151
- Format
- –
- Schlagworte
- Antigens, CD34 - metabolism, Cell Cycle, Cell Differentiation, Cell Lineage, Cell Shape, Cytochalasin D - pharmacology, Cytoskeleton, Equipment Design, Flow Cytometry - instrumentation, Flow Cytometry - methods, Hematopoietic Stem Cells - cytology, Hematopoietic Stem Cells - metabolism, HL-60 Cells - cytology, HL-60 Cells - drug effects, Humans, Microfluidic Analytical Techniques