Details

Autor(en) / Beteiligte

• Dutov, A A
• Nikitin, D A
• Luk'ianova, Yu L
• Sverkunova, A V
• Martynova, A V
• Ermolina, A V

Titel

HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (Purosep-200)

Ist Teil von

• Biomeditsinskaia khimiia, 2014-11, Vol.60 (6), p.651

Ort / Verlag

Russia (Federation)

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• We have developed a simple HPLC method for analysis of the dehydroepiandrosterone sulfate (DHEA-sulfate) in serum with use a new procedure of solid-phase extraction (SPE) on hyper cross-linked polystyrene (Purosep-200) and fast chromatographic separation on the monolithic column under isocratic elution and UV detection at 200 nm. Complete SPE procedure lasts for about 7 min, chromatographic separation takes less than 6 min. Simplicity and high reproducibility of this method makes it attractive in routine clinical practice.