Details

Autor(en) / Beteiligte

• Sugahara, Michihiro
• Mizohata, Eiichi
• Nango, Eriko
• Suzuki, Mamoru
• Tanaka, Tomoyuki
• Masuda, Tetsuya
• Tanaka, Rie
• Shimamura, Tatsuro
• Tanaka, Yoshiki
• Suno, Chiyo
• Ihara, Kentaro
• Pan, Dongqing
• Kakinouchi, Keisuke
• Sugiyama, Shigeru
• Murata, Michio
• Inoue, Tsuyoshi
• Tono, Kensuke
• Song, Changyong
• Park, Jaehyun
• Kameshima, Takashi
• Hatsui, Takaki
• Joti, Yasumasa
• Yabashi, Makina
• Iwata, So

Titel

Grease matrix as a versatile carrier of proteins for serial crystallography

Ist Teil von

• Nature methods, 2015-01, Vol.12 (1), p.61-63

Ort / Verlag

United States

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.