Methods in molecular biology (Clifton, N.J.), 2015-01, Vol.1229, p.567
2015
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- Autor(en) / Beteiligte
- Titel
- Role of glycosaminoglycans in infectious disease
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2015-01, Vol.1229, p.567
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Glycosaminoglycans (GAGs) have been shown to bind to a wide variety of microbial pathogens, including viruses, bacteria, parasites, and fungi in vitro. GAGs are thought to promote pathogenesis by facilitating pathogen attachment, invasion, or evasion of host defense mechanisms. However, the role of GAGs in infectious disease has not been extensively studied in vivo and therefore their pathophysiological significance and functions are largely unknown. Here we describe methods to directly investigate the role of GAGs in infections in vivo using mouse models of bacterial lung and corneal infection. The overall experimental strategy is to establish the importance and specificity of GAGs, define the essential structural features of GAGs, and identify a biological activity of GAGs that promotes pathogenesis.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-4939-1714-3_45Titel-ID: cdi_pubmed_primary_25325982
- Format
- –
- Schlagworte
- Administration, Intranasal, Animals, Antimicrobial Cationic Peptides - pharmacology, Communicable Diseases - metabolism, Communicable Diseases - microbiology, Communicable Diseases - pathology, Cornea - drug effects, Cornea - microbiology, Cornea - pathology, Glycosaminoglycans - antagonists & inhibitors, Glycosaminoglycans - metabolism, Heparitin Sulfate - pharmacology, Lung - drug effects, Lung - microbiology, Lung - pathology, Mice, Inbred BALB C, Mice, Knockout, Microbial Sensitivity Tests, Microbial Viability - drug effects, Pseudomonas aeruginosa - drug effects, Pseudomonas Infections - microbiology, Pseudomonas Infections - pathology, Staphylococcal Infections - microbiology, Staphylococcal Infections - pathology, Staphylococcus aureus - drug effects, Syndecan-1 - metabolism