Details

Autor(en) / Beteiligte

• Liang, Chien-Fu
• Hahm, Heung Sik
• Seeberger, Peter H

Titel

Automated synthesis of chondroitin sulfate oligosaccharides

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2015-01, Vol.1229, p.3

Ort / Verlag

United States

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Glycosaminoglycans (GAGs) are important sulfated carbohydrates prevalently found in the extracellular matrix that serve many biological functions. The synthesis of structurally diverse but defined GAGs is extremely challenging as one has to account for the various sulfation patterns. Described is the automated synthesis of two chondroitin sulfate hexasaccharides. The oligosaccharides are prepared on a solid support that is equipped with a photolabile linker. The linker cleavage from the resin is performed in a continuous-flow photoreactor under chemically mild conditions. The described approach will serve as a general scheme to systematically access oligosaccharides of all GAG families.