Details

Autor(en) / Beteiligte

• Clayton, David A
• Shadel, Gerald S

Titel

Isolation of mitochondria from tissue culture cells

Ist Teil von

• Cold Spring Harbor protocols, 2014-10, Vol.2014 (10), p.pdb.prot080002

Ort / Verlag

United States

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The number of mitochondria per cell varies substantially from cell line to cell line. For example, human HeLa cells contain at least twice as many mitochondria as smaller mouse L cells. This protocol starts with a washed cell pellet of 1-2 mL derived from ∼10⁹ cells grown in culture. The cells are swollen in a hypotonic buffer and ruptured with a Dounce or Potter-Elvehjem homogenizer using a tight-fitting pestle, and mitochondria are isolated by differential centrifugation.