Details

Autor(en) / Beteiligte

• Nannenga, Brent L
• Shi, Dan
• Leslie, Andrew G W
• Gonen, Tamir

Titel

High-resolution structure determination by continuous-rotation data collection in MicroED

Ist Teil von

• Nature methods, 2014-09, Vol.11 (9), p.927

Ort / Verlag

United States

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology.