Details

Autor(en) / Beteiligte

• Lu, Xue-Ying
• Cao, Wei-Ke
• Ye, Li-Lin
• Deng, Zhi-Kui
• Zhang, Xiao-Hui
• Li, Yu-Feng

Titel

Time rhythm of homoharringtonine inducing K562 cell apoptosis and its mechanism

Ist Teil von

• Zhongguo shi yan xue ye xue za zhi, 2014-06, Vol.22 (3), p.712

Ort / Verlag

China

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• This study was aimed to explore the change of K562 cell apoptosis at different time point after homoharringtonine (HHT) treatment and its mechanism. After treatment of K562 cells with 10 ng/ml HHT, the cell viability was tested with MTT assay; the expression of caspase-3 was detected with Western blot; the BCL-2 expression was analyzed with flow cytometry; the autophagosome was observed by electron microscopy. The results showed that the viability of K562 cells reduced gradually from day 1 to day 5 and ascended from day 6 to day 8 after HHT treatment. At the same time, the cleaved caspase-3 expression level of K562 cells increased gradually from day 1 to day 7, but reduced at the day 8 (P < 0.05). From day 1 to day 8 after HHT treatment, the BCL-2 expression level declined firstly and then went up (P < 0.05). Autophagosome was also seen remarkably at day 8 after HHT treatment. It is concluded that the apoptosis level of K562 cells after being treated with HHT enhances firstly and then declines , which may be