Details

Autor(en) / Beteiligte

• Zhao, Huan-Ge
• Fan, Zhi-Gang
• Huang, Feng-Ying
• Huang, Yong-Hao
• Zhou, Song-Lin
• Lin, Ying-Ying
• Tan, Guang-Hong

Titel

Immune response in mice induced by complex gene vaccine pcSAG1-ROP5 of Toxoplasma gondii

Ist Teil von

• Zhongguo jishengchongxue yu jishengchongping zazhi, 2013-02, Vol.31 (1), p.1

Ort / Verlag

China

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• To observe the immune response induced by complex gene vaccine pcSAG1-ROP5 of Toxoplasma gondii in mice. The recombinant eukaryotic expression plasmids pcSAG1, pcROP5 and pcSAG1-ROP5 were constructed and identified by PCR, restriction enzyme digestion, and sequencing. The three recombinant plasmids were transfected into HeLa cells to express in vitro and identified by Western blotting analysis. Seventy Kunming mice were randomly divided into 5 groups with 14 each, i.e. pcSAG1 group, pcROP5 group, pcSAG1-ROP5 group, blank plasmid group and PBS control group. The mice were immunized intramuscularly with pcSAG1, pcROP5, pcSAG1-ROP5, pcDNA3.1, and PBS, respectively, every two weeks for three times. Sera were collected before each injection and 2 weeks after the last immunization. The titer of mice serum in pcSAG1-ROP5 group combined with recombinant protein SAG1, ROP5 and SAG1-ROP5 and the level of IgG against T. gondii in 5 groups were determined by ELISA. Three weeks after the last immunization, ten mice of eac