Details

Autor(en) / Beteiligte

• Liu, Gaoxia
• Fan, Mingwen
• Guo, Jihua

Titel

Efficacy of immune responses induced by anti-caries DNA vaccine-loaded bacterial ghost in mice

Ist Teil von

• Zhonghua kouqiang yixue zazhi, 2014-01, Vol.49 (1), p.37

Ort / Verlag

China

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• To develop an anti-caries DNA vaccine-loaded Salmonella typhimurium (St) ghost and enhance the efficacy of immune responses induced by anti-caries DNA vaccine via mucosal route. Both pREP4 and PhiX gene E expression plasmids were transformed into StJ357 and then induced with isopropyl β-D-1-thiogalactopyranoside (IPTG). The bacterial ghosts (BG) were collected after wash and loaded with anti-caries DNA vaccine pGJGLU/VAX. Mice were divided into four groups and immunized through the nasal route with pGJGLU/VAX-loaded BG (Group Ghost+pGJGLU/VAX), pVAX1-loaded BG (Group Ghost+pVAX1), pGJGLU/VAX-Bupivacaine complex (Group pGJGLU/VAX) and pVAX1-Bupivacaine complex (Group pVAX1), respectively. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the immune responses. ELISA results showed that group Ghost+pGJGLU/VAX had significantly higher level of specific anti-GLU SIgA antibody [(0.367 ± 0.086) A/µg] compared with group Ghost+pVAX1 [(0.122 ± 0.077) A/µg], Group pGJGLU/VAX[(0.068 ± 0.068) A/µg] or Group