Methods in molecular biology (Clifton, N.J.), 2014, Vol.1105, p.623
2014
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Details
- Autor(en) / Beteiligte
- Titel
- Detection of programmed cell death in cells exposed to genotoxic agents using a caspase activation assay
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2014, Vol.1105, p.623
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Many toxins that individuals are exposed to cause DNA damage. Cells that have sustained DNA damage may attempt to repair the damage prior to replication. However, if a cell has sustained serious damage it cannot repair, it will commit suicide through a genetically regulated programmed cell death (PCD) pathway. Crucial to the ultimate execution of PCD is a family of cysteine proteases called caspases. Activation of these enzymes occurs late enough in the PCD pathway that a cell can no longer avoid cell death, but still earlier than PCD-associated morphological changes or DNA fragmentation. This protocol details a method for using fluorochrome-conjugated caspase inhibitors for the detection of activated caspases in intact cells. The analysis and documentation is performed using fluorescence microscopy.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-62703-739-6_43Titel-ID: cdi_pubmed_primary_24623257
- Format
- –
- Schlagworte
- Animals, Apoptosis, Caspase Inhibitors - chemistry, Caspases - metabolism, Cells, Cultured, DNA Damage, Enzyme Activation, Enzyme Assays, Fluoresceins - chemistry, Fluorescent Dyes - chemistry, Humans, Microscopy, Fluorescence, Mutagens - toxicity, Staining and Labeling, Tissue Fixation