Details

Autor(en) / Beteiligte

• Romakhina, E R
• Bagaeva, T V
• Sharipova, F R
• Kozyreva, E A
• Leshchinskaia, I B

Titel

Isolation and study of polyadenylated RNA from Bacillus intermedius

Ist Teil von

• Mikrobiologija (Moskva. 1932), 1988-05, Vol.57 (3), p.421

Ort / Verlag

Russia (Federation)

Erscheinungsjahr

1988

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• A poly(A)+RNA fraction was isolated from the overall RNA of Bacillus intermedius using chromatography on poly(U) Sepharose and was shown to be electrophoretically heterogeneous. The presence of a polyadenylate segment was confirmed by hybridization with polyuridine. The biological activity of the poly(A)+RNA was proved by the translation in Xenopus laevis oocytes. The dynamics of poly(A)+RNA synthesis was studied in the course of B. intermedius growth and the content of poly(A)+RNA was assayed in the cells grown in different media.