Details

Autor(en) / Beteiligte

• Hoyuelos, Francisco J
• García, Begoña
• Leal, José M
• Busto, Natalia
• Biver, Tarita
• Secco, Fernando
• Venturini, Marcella

Titel

RNA triplex-to-duplex and duplex-to-triplex conversion induced by coralyne

Ist Teil von

• Physical chemistry chemical physics : PCCP, 2014-04, Vol.16 (13), p.612-618

Ort / Verlag

England

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Spectrophotometric, circular dichroism, calorimetric, displacement assay and kinetic analyses of the binding of the fluorescent dye coralyne to poly(A)2poly(U) have served to enlighten the ability of the dye to produce dramatic changes in the RNA structure. The sets of data assembled convey that coralyne is able to induce the triplex-to-duplex conversion and also the duplex-to-triplex conversion according to a non-reversible cycle governed by temperature, provided that the [dye]/[polymer] ratio ( C D / C P ) is maintained constant above unity. Alternatively, at room temperature the triplex is formed at (roughly) C D / C P < 1 and the duplex at C D / C P > 1. RNA cyclic transitions by coralyne for C D / C P > 1.