Details

Autor(en) / Beteiligte

• Chen, Zhi-bin
• Lin, Qin
• Ma, Chang-hua
• Liu, Kai-ning
• Meng, Huan-xin

Titel

Characteristic of sample banks isolated from EDTA-blood by sedimentation method

Ist Teil von

• Beijing da xue xue bao. Journal of Peking University. Yi xue ban, 2014-02, Vol.46 (1), p.111

Ort / Verlag

China

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• To assess the characteristics of establishing the different sample banks of plasma, leukocytes and DNA by sedimentation method of isolating from ethylene diamine tetraacetic acid(EDTA)-blood and to clarify the sedimentation method of leukocyte isolation and plasma volume by comparative data and recommended procedures for applicability. In the study, 29 EDTA-bloods were obtained, the total amounts of leukocytes and the percentage of neutrophile granulocytes, and lymphocytes in the EDTA-blood detected as a control group and then assigned equally into 4 EP tubes with 1 mL EDTA-blood per tube as 4 test groups, then the 4 tubes were placed with the EDTA-blood at room temperature and the plasma layers were isolated at 0.5, 1, 2 and 3 h, receptively. The total amount of leukocytes and the percentage of neutrophile granulocytes, and lymphocytes were detected by automated hematology analyzer at the clinical laboratory. The volume of the plasma was also measured at the same time. The plasma volume at 0.5 h [(241.72 ± 1