Details

Autor(en) / Beteiligte

• Liu, Bianjiang
• Tang, Min
• Han, Zhijian
• Li, Jie
• Zhang, Jiexiu
• Lu, Pei
• Song, Ninghong
• Wang, Zengjun
• Yin, Changjun
• Zhang, Wei

Titel

Co-Incubation of Human Spermatozoa with Anti-VDAC Antibody Reduced Sperm Motility

Ist Teil von

• Cellular physiology and biochemistry, 2014-01, Vol.33 (1), p.142-150

Ort / Verlag

Basel, Switzerland: Cell Physiol Biochem Press GmbH & Co KG

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Background: Voltage-dependent anion channel (VDAC), a channel protein, exists in the outer mitochondrial membrane of somatic cells and is involved in multiple physiological and pathophysiological processes. Up until now, little has been known about VDAC in male germ cells. In the present study, the relationship between VDAC and human sperm motility was explored. Methods: Highly motile human spermatozoa were incubated in vitro with anti-VDAC antibody. Total sperm motility, straight line velocity (VSL), curvilinear velocity (VCL), and average path velocity (VAP) were recorded. Intracellular free calcium concentration ([Ca2+]i), pH value (pHi), and ATP content were determined. Results: Co-incubation with anti-VDAC antibody reduced VSL, VCL, and VAP of spermatozoa. Co-incubation further reduced [Ca2+]i. Anti-VDAC antibody did not significantly alter total sperm motility, pHi and intracellular ATP content. Conclusion: The data suggest that co-incubation with anti-VDAC antibody reduces sperm motility through inhibition of Ca2+ transmembrane flow. In this way, VDAC participates in the modulation of human sperm motility through mediating Ca2+ transmembrane transport and exchange.