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The purpose of this guideline is to provide comprehensive state-of-the-art information about indication and how to perform and analyze bone scintigraphy. Based upon pathophysiology and pharmacology current acquisition techniques including new methodologies are summarized followed by a detailed list of indications. In the main part all relevant practical aspects such as patient preparation, anamnestic information, appropriate choice and dosage of the radiopharmaceutical, and data acquisition including interventions are discussed. Data processing and analysis, interpretation, reporting and documentation are described in the next chapters. Quality control, typical pitfalls and a short outlook to future developments complete the guideline.