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Neuropeptides degranulate serous cells of ferret tracheal glands
Ist Teil von
American Journal of Physiology: Cell Physiology, 1986-08, Vol.251 (2), p.C223-C229
Ort / Verlag
United States
Erscheinungsjahr
1986
Quelle
MEDLINE
Beschreibungen/Notizen
A. A. Gashi, D. B. Borson, W. E. Finkbeiner, J. A. Nadel and C. B. Basbaum
To determine whether serous or mucous cells in tracheal submucosal glands
respond to the neuropeptides substance P (SP) and vasoactive intestinal
peptide (VIP), we studied the peptide-induced changes in gland cell
morphology accompanying release of 35SO4-labeled macromolecules from
tracheal explants of ferrets. Explants were labeled for 1 h in medium
containing 35SO4 and washed for 3.5 additional hours. Base-line secretion
in the absence of drugs declined between 1.5 and 3.5 h after the pulse.
Between 2.5 and 3.5 h, the average percent change in counts per minute
recovered per sample period was not significantly different from zero (P
greater than 0.3; n = 6). Substance P (10(-5) M) and VIP (2 X 10(-6) M)
added 4 h after labeling each increased greatly the release of
35SO4-labeled macromolecules (SP, 219%; VIP, 180%) above base line.
Bethanechol, a muscarinic-cholinergic agonist (10(-5) M), increased
secretion by an average of 142% above base line (each effect, P less than
0.05; n = 6 each). Light and electron microscopy of the control tissues
showed glands with narrow lumens and numerous secretory granules. Glands
treated with SP or VIP had enlarged lumens and the serous cells were
markedly degranulated. These phenomena were documented by morphometry and
suggest that SP and VIP cause secretion from glands at least partially by
stimulating exocytosis from serous cells.