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Monitoring of transcriptional dynamics of HIF and NFκB activities
Ist Teil von
Methods in molecular biology (Clifton, N.J.), 2014, Vol.1098, p.97
Ort / Verlag
United States
Erscheinungsjahr
2014
Quelle
MEDLINE
Beschreibungen/Notizen
Genetic experiments over the last few decades have identified many regulatory proteins critical for DNA transcription. The dynamics of their transcriptional activities shape the differential expression of the genes they control. Here we describe a simple method, based on the secreted luciferase, to measure the activities of two transcription factors NFκB and HIF. This technique can effectively monitor dynamics of transcriptional events in a population of cells and be up-scaled for high-throughput screening and promoter analysis, making it ideal for data-demanding applications such as mathematical modelling.