Details

Autor(en) / Beteiligte

• Zhang, Yong
• Lai, Zhifa
• Zhou, Haitao
• Li, Bo
• Chen, Runli
• Zeng, Huashu
• Hou, Hongbin

Titel

Study on the serotype, virulence genes and pulsed field gel electrophoresis molecular typing of Vibrio parahaemolyticus isolated from clinical patients

Ist Teil von

• Wei sheng yan jiu, 2013-07, Vol.42 (4), p.619

Ort / Verlag

China

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the serotype virulence genes and molecular typing characteristics of Vibrio Parahaemolyticus isolated from diarrhea samples among 2007 -2012. The serotype was detected by using serological agglutination test. Thermo-stable direct hemolysin (tdh) and tdh-related hemolysin (trh) were detected by using real time PCR methods. 56 strains 03:K6 were analyzed for molecular typing by pulsed field gel electrophoresis(PFGE). (1) There are 14 serotypes, including 03: K6 (114,67.9%),04: K8 (25,14.9%), 03: K29 (6,3.6%) and et al. Compared with the serotypes of strains from food poison, these from sporadic cases with diarrhea were different, but the main serotype was also 03:K6. (2) There are 4 types of virulence genes, including tdh+ trh- (161, 95.8%), tdh+ trh+ (3,1.8%), tdh- trh+ (1, 0.6%) tdh- trh- (3, 1.8%). Compared with the virulence genes of strains from food poison, these from sporadic cases with diarrhea were different, but the main virulence gene was also tdh+ trh-. 3,56 strains belonged to 15 PFG