Zhōnghuá zhŏngliú zázhì, 2013-03, Vol.35 (3), p.164
2013
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- Autor(en) / Beteiligte
- Titel
- Morphological observation of human gastric cancer cell SGC-7901 clones and identification of gastric cancer stem cells
- Ist Teil von
- Zhōnghuá zhŏngliú zázhì, 2013-03, Vol.35 (3), p.164
- Ort / Verlag
- China
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To dynamically investigate the morphology of human gastric cancer SGC-7901 cell clones, and then compare the tumorigenic ability of different clones in order to identify the tumor stem cell clones. Clones derived from gastric cancer SGC-7901 cells were assessed by morphological observation, and the clone formation rate and proportion of each clone were calculated. The expression of CD44 and CDX2 in different clones was detected by immunofluorescence microscopy and Western blot. Furthermore, different clones were isolated and cultured, and their self-renewal property was assayed. Cells of different clones were subcutaneously inoculated into nude mice and the tumorigenic ability of each group was determined. Clones derived from gastric cancer SGC-7901 cells had three types, i.e. clones of tight, transitional and loose types. The total clone formation rate was (9.80 ± 1.07)%, and the proportion of tight, transitional and loose type clones was 10.2%, 56.0% and 33.8%, respectively. The results of immunofluorescenc
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0253-3766DOI: 10.3760/cma.j.issn.0253-3766.2013.03.002Titel-ID: cdi_pubmed_primary_23879994
- Format
- –
- Schlagworte
- Animals, CDX2 Transcription Factor, Cell Differentiation, Cell Line, Tumor, Cell Proliferation, Clone Cells - classification, Female, Homeodomain Proteins - metabolism, Humans, Hyaluronan Receptors - metabolism, Mice, Mice, Nude, Neoplasm Transplantation, Neoplastic Stem Cells - cytology, Neoplastic Stem Cells - metabolism, Random Allocation, Stomach Neoplasms - metabolism, Stomach Neoplasms - pathology