Details

Autor(en) / Beteiligte

• Snider, Jamie
• Hanif, Asad
• Lee, Mid Eum
• Jin, Ke
• Yu, Analyn R
• Graham, Chris
• Chuk, Matthew
• Damjanovic, Dunja
• Wierzbicka, Marta
• Tang, Priscilla
• Balderes, Dina
• Wong, Victoria
• Jessulat, Matthew
• Darowski, Katelyn D
• San Luis, Bryan-Joseph
• Shevelev, Igor
• Sturley, Stephen L
• Boone, Charles
• Greenblatt, Jack F
• Zhang, Zhaolei
• Paumi, Christian M
• Babu, Mohan
• Park, Hay-Oak
• Michaelis, Susan
• Stagljar, Igor

Titel

Mapping the functional yeast ABC transporter interactome

Ist Teil von

• Nature chemical biology, 2013-09, Vol.9 (9), p.565

Ort / Verlag

United States

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• ATP-binding cassette (ABC) transporters are a ubiquitous class of integral membrane proteins of immense clinical interest because of their strong association with human disease and pharmacology. To improve our understanding of these proteins, we used membrane yeast two-hybrid technology to map the protein interactome of all of the nonmitochondrial ABC transporters in the model organism Saccharomyces cerevisiae and combined this data with previously reported yeast ABC transporter interactions in the BioGRID database to generate a comprehensive, integrated 'interactome'. We show that ABC transporters physically associate with proteins involved in an unexpectedly diverse range of functions. We specifically examine the importance of the physical interactions of ABC transporters in both the regulation of one another and in the modulation of proteins involved in zinc homeostasis. The interaction network presented here will be a powerful resource for increasing our fundamental understanding of the cellular role and regulation of ABC transporters.