Details

Autor(en) / Beteiligte

• Yuan, Yi
• Lu, Feng
• Gao, Jianhua

Titel

Mechanism of vascular stromal fraction at early stage after aspirated fat transplantation

Ist Teil von

• Zhongguo xiu fu chong jian wai ke za zhi, 2013-04, Vol.27 (4), p.449

Ort / Verlag

China

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the mechanism of vascular stromal fraction (SVF) at the early stage after aspirated fat transplantation. Fat was harvested from 5 cases of women undergoing abdominal liposuction operation, and SVF was isolated. Aspirated fat with (group B) or without (group A) SVF was injected subcutaneously into the back of nude mice, and the grafts were harvested at 1, 3, 5, and 7 days. Graft wet weight was measured; and immunohistochemical method (CD31) was performed and the secretion of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were qnantified by Western blot assay. The wet weight of transplanted adipose tissue showed an increasing tendency in groups A and B with time, and no significant difference was found between groups A and B (P > 0.05). At 1 and 3 days after transplantation, no CD31 positive cells was seen in 2 groups; the CD31 positive cells of group B were significantly more than those of group A at 5 and 7 days (P < 0.05), and the CD31 positive cells at 7 days wer