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Localization and function of CD59 and Cbp in T lymphocytes
Ist Teil von
Xi bao yu fen zi mian yi xue za zhi, 2013-06, Vol.29 (6), p.565
Ort / Verlag
China
Erscheinungsjahr
2013
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
To investigate the distributions of GPI-anchored protein CD59 and C-terminal Src kinase-binding protein (Cbp) in cell membrane of T lymphocytes and the roles in cell activation and proliferation.
The locations of CD59 and Cbp were observed under a fluorescence microscope with the immunofluorescence cytochemistry. The Jurkat cells were transfected with the recombinant plasmid pSUPER-siCD59 using the electric transfection method. Using RT-PCR and Western blotting, we detected the expression of CD59 and the phosphorylation level of protein-tyrosine kinase oncogene family (fyn) in different groups. Moreover, the cell proliferation activity was measured by MTT assay.
The immunofluorescence cytochemistry showed that CD59 and Cbp were mainly distributed in cell membrane. Compared with other groups, the quantitative RT-PCR and Western blotting showed that the expression of CD59 was reduced, and along with it the phosphorylation level of fyn decreased in pSUPER-siCD59 group, and MTT assay revealed that its cell prolif