Details

Autor(en) / Beteiligte

• Dorier, Julien
• Stasiak, Andrzej

Titel

Modelling of crowded polymers elucidate effects of double-strand breaks in topological domains of bacterial chromosomes

Ist Teil von

• Nucleic acids research, 2013-08, Vol.41 (14), p.6808

Ort / Verlag

England

Erscheinungsjahr

2013

Link zum Volltext

Beschreibungen/Notizen

• Using numerical simulations of pairs of long polymeric chains confined in microscopic cylinders, we investigate consequences of double-strand DNA breaks occurring in independent topological domains, such as these constituting bacterial chromosomes. Our simulations show a transition between segregated and mixed state upon linearization of one of the modelled topological domains. Our results explain how chromosomal organization into topological domains can fulfil two opposite conditions: (i) effectively repulse various loops from each other thus promoting chromosome separation and (ii) permit local DNA intermingling when one or more loops are broken and need to be repaired in a process that requires homology search between broken ends and their homologous sequences in closely positioned sister chromatid.