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Inhibiting effect of IL-10 in tumor microenvironment on anti-tumor activity of SOCS1-silenced DC vaccine
Ist Teil von
Xi bao yu fen zi mian yi xue za zhi, 2013-04, Vol.29 (4), p.379
Ort / Verlag
China
Erscheinungsjahr
2013
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
To observe the anti-tumor effect of suppressors of cytokine signaling 1(SOCS1)-silenced dentritic cell (DC) vaccines in melanoma-bearing mice, and the influence of IL-10 in the tumor microenvironment on DC vaccine action.
To obtain SOCS1-silenced DCs, DCs derived from mouse bone marrow cells ex vivo were induced to differentiation in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4, and then transduced with Len-SOCS1-shRNA or control Len-GFP lentiviruses. The SOCS1-silenced DCs were loaded by TRP2 peptide to prepare the DC vaccine, which was induced to mature by LPS. The DCs were analyzed by flow cytometry (FCM) for surface expressions of MHCII and CD86 and by real-time PCR for the expressions of SOCS1, IL-10 as well as IL-12p40. B16 or IL-10-silenced B16 (IL-10(-/-);) cells were inoculated into C57BL/6 mice. Five days later, the mice were randomly divided into 3 groups (PBS-DC, Len-DC and SOCS1-shRNA-DC groups) and injected with 1×10⁶;/100 μL per mouse of the transduced DCs