Details

Autor(en) / Beteiligte

• Shaner, Nathan C
• Lambert, Gerard G
• Chammas, Andrew
• Ni, Yuhui
• Cranfill, Paula J
• Baird, Michelle A
• Sell, Brittney R
• Allen, John R
• Day, Richard N
• Israelsson, Maria
• Davidson, Michael W
• Wang, Jiwu

Titel

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

Ist Teil von

• Nature methods, 2013-05, Vol.10 (5), p.407-409

Ort / Verlag

United States

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.