Methods in molecular biology (Clifton, N.J.), 2013, Vol.977, p.259
2013
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Peptide microarrays for profiling of serine/threonine kinase activity of recombinant kinases and lysates of cells and tissue samples
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2013, Vol.977, p.259
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Peptide microarray technology can be used to identify substrates for recombinant kinases, to measure kinase activity and changes thereof in cell lysates and lysates from fresh frozen (tumor) tissue. The effect of kinase inhibitors on the kinase activities in relevant tissues can be investigated as well. The method for performing experiments on dynamic peptide microarrays with real-time readout is described, as well as the influence of assay parameters and suggestions for optimization of experiments.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-62703-284-1_21Titel-ID: cdi_pubmed_primary_23436369
- Format
- –
- Schlagworte
- Animals, Cell Extracts, Cell Fractionation, Enzyme Assays, Humans, Immobilized Proteins - chemistry, Kinetics, MCF-7 Cells, Peptide Fragments - chemistry, Phosphorylation, Protein Array Analysis - methods, Protein Processing, Post-Translational, Protein-Serine-Threonine Kinases - chemistry, Recombinant Proteins - chemistry