Details

Autor(en) / Beteiligte

• Zhu, Yan-ju
• Pan, Yue
• Chen, Jun-ying
• Ma, Zhong-fei
• Deng, Xin-qiang
• Liu, Jian-sheng
• Ma, Shao-hui

Titel

Complete nucleotide sequence of a human echovirus 6 strain KM57-09 isolated in Yunnan, China, in 2009

Ist Teil von

• Zhōnghuá liúxíngbìng zázhì, 2012-09, Vol.33 (9), p.951

Ort / Verlag

China

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• To analyze the genetic characterization of the complete genome from a human echovirus 6 (Echo6) strain KM57-09 isolated in Yunnan, China, in 2009. Using the RT-PCR, eight fragments containing about 1000 nucleotides which covered the whole viral genome were sequenced. The sequences were aligned with other reference enterovirus sequences downloaded from the GenBank, using Mega 5.05, RDP 3 and SimPlot 3.5.1 softwares. Similar to the other human enterovirus, KM57-09 isolate genome appeared to have 7419 nucleotides in length, encoding for 2191 amino acids. In the complete genome, the rates of homology on nucleotide and amino acid among the seven Echo6 isolates were 79.3% - 80.2% and 93.3% - 94.4%, respectively as well as 79.3% and 93.6% of the rates of homology when compared with that of D' Amor prototype strain. In different segment of genome. The 2C-3A genome region was most similar to the HN-2-E25 strain, the 5' UTR, VP4, 3D and 3' UTR genome region were most similar to the CoxB5-Henan-2010. In the VP1 gene, th