Details

Autor(en) / Beteiligte

• Chihota, V N
• van Halsema, C L
• Grant, A D
• Fielding, K L
• van Helden, P D
• Churchyard, G J
• Gey van Pittius, N C

Titel

Spectrum of non-tuberculous mycobacteria identified using standard biochemical testing vs. 16S sequencing

Ist Teil von

• The international journal of tuberculosis and lung disease, 2013-02, Vol.17 (2), p.267-269

Ort / Verlag

France

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Non-tuberculous mycobacterial isolates from gold miners were speciated using standard biochemical testing (SBT) and 16S rDNA sequencing. Of 237 isolates tested, SBT identified 126, compared with all 237 identified using sequencing. Of 111 isolates unspeciated by SBT but identified by sequencing, 38 (34.2%) were identified as Mycobacterium gordonae and 8 (7.2%) were new species. Of 126 isolates speciated by both methods, 37 were discordant, with 14/17 M. gordonae isolates incorrectly identified as M. scrofulaceum using SBT. The majority of these were the potentially pathogenic strain D, M. gordonae. Sequencing is preferable where available to guide treatment.