Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery, 2012-09, Vol.47 (9), p.749
2012
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Details
- Autor(en) / Beteiligte
- Titel
- EGCG regulates TGF-β1-induced epithelial mesenchymal transition in squamous cell carcinoma of head and neck
- Ist Teil von
- Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery, 2012-09, Vol.47 (9), p.749
- Ort / Verlag
- China
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To study the effect and molecular mechanism of epigallocatechin-3-gallate (EGCG) on epithelial-mesenchymal transition (EMT) in vitro induced by human recombinant TGF-β1 protein in squamous cell carcinoma of the head and neck. EMT morphological changes of Tu686 cells were observed after sequential treatment of 5 ng/ml TGF-β1 and 20 µmol/L EGCG. Tu686 cells were collected after the treatment of 5 ng/ml TGF-β1 for 24 h and EGCG with different concentrations (0, 10, 20, 30 µmol/L) for another 24 h or 20 µmol/L EGCG treatment for different time phase (6, 12, 24 h). Then RT-PCR and Western-blot were applied to detect mRNA and protein expression level of epithelial cell marker E-cadherin, mesenchymal cell marker Vimentin and Smad7, an inhibit molecule of TGF-β1 mediated pathway in Tu686 cells. TGF-β1 successfully induced characterized EMT morphological and molecular changes in Tu686 cells, in which expression of E-cadherin decreased, Vimentin increased and Smad7 declined. However, EGCG could reverse the TGF-β1 media
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1673-0860Titel-ID: cdi_pubmed_primary_23141445
- Format
- –
- Schlagworte
- Antigens, CD, Cadherins - metabolism, Carcinoma, Squamous Cell - metabolism, Catechin - analogs & derivatives, Catechin - pharmacology, Cell Line, Tumor, Epithelial-Mesenchymal Transition - drug effects, Head and Neck Neoplasms - metabolism, Humans, Signal Transduction - drug effects, Smad7 Protein - metabolism, Squamous Cell Carcinoma of Head and Neck, Transforming Growth Factor beta1 - metabolism, Vimentin - metabolism