Details

Autor(en) / Beteiligte

• Ren, Jing
• Lei, Lin-Sheng
• Yu, Chuan-Lin
• He, Gai-Yi-Nan
• Chen, Na-Na

Titel

Study on the anti-endotoxin effect of saponin from Tupistra chinensis

Ist Teil von

• Zhong yao cai, 2012-01, Vol.35 (1), p.102

Ort / Verlag

China

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the effect of saponin from Tupistra chinensis Baker (STCB) on lethal toxicity of endotoxin in mice and explore the underlying mechanism. Mouse models of endotoxin-induced death and endotoximia were established by intraperitoneal administration of KM mice with lipopolysaccharides (LPS) from Pseudomonas aeruginosa in doses of 60 mg/kg and 10 mg/kg respectively. Mouse survival rate and survival time were recorded and the serum levels of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) in endotoximia mice were measured by enzyme-linked immunosorbent assay (ELISA). Mouse peritoneal exudate cells induced by LPS were used as an in vitro inflammatory model,which was then intervened with STCB and the levels of IL-1beta and TNF-alpha in the culture supernatants were measured by ELISA. The survival rates of mice prophylactically treated with STCB (200 and 400 mg/kg, in 5 consecutive days) were slightly higher compared with that in model group,but no statistical difference was obser